Novel insights into fluorescent ‘dark states’ illuminate ways forward for improved imaging
Scientists at St. Jude Children’s Research Hospital today announced a way to improve molecular scale distance measurements using single-molecule fluorescence resonance energy transfer (smFRET). smFRET quantifies the excitation and emission properties of chemicals called fluorophores. When an excited electron in the fluorophore relaxes, it emits light after a delay, causing the molecule to glow (fluoresce). … Read more